Title: High Resolution Chromatography: A Practical Approach (Practical Approach Series)
Publisher: Oxford University Press, U.S.A.
Publication Date: 1999
Language: English
ISBN 10: 0199636486
ISBN 13: 9780199636488
Binding: Soft cover
Edition: 1st Edition
Condition: Very Good

About this title

Synopsis

Isolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques, detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.

About the Author

Paul Millner, School of Biochemistry and Molecular Biology, University of Leeds.

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