Nuclear Receptors : A Practical Approach

The steroid or nuclear receptor superfamily is an important group of transcription factors that is studied by a large and varied number of basic and clinical researchers. Nuclear Receptors: A Practical Approach provides these researchers an invaluable guide to do so. With an international list of contributors and examples of over 70 tried and true protocols, this book offers substantial insight into the growing family of biologically important receptors. Its chapters address a variety of factors, including the evolutionary biology of the superfamily and how to clone and characterize new receptors. Also addressed are topics such as identification of the ligands of novel receptors, the kinetic analysis of receptor interactions, and the functional characterization of coactivators using microinjection. Various sections throughout the book cover receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. The book concludes with a description of yeast as a model system for looking at receptor function, and the final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a useful tool for any researcher studying members of the superfamily, and it is a must for all biomedical researchers.

Didier Picard is at University of Geneva.