PRINS and In Situ PCR Protocols (Methods in Molecular Biology: Volume 71)

Gosden, John R.

ISBN 10: 0896033953 / ISBN 13: 9780896033955

Published by Humana Press, Totowa, New Jersey

About this Item

This softcover edition of "PRINS and In Situ PCR Protocols (Methods in Molecular Biology: Volume 71)" by John R. Gosden is in excellent condition, rated as Near Fine/VG+. The covers and the plastic comb binding are well-preserved, with the binding remaining intact and free from any breaks. Although there is an organization stamp on the half-title page, it has been neatly marked out. The interior pages are in pristine condition, appearing clean, bright, and free of markings. The book will be packaged with care to ensure its protection during shipment, and an USPS electronic tracking number will be provided at no extra cost. If you would like more information or additional pictures of the book, please don't hesitate to contact me.About the Book:This book presents the work of cutting-edge researchers, offering step-by-step instructions on how to effectively use oligonucleotide primers for the detection, amplification, or extension of complementary sequences in situ. The techniques outlined in this book, many of which were developed by the authors themselves, provide new opportunities for the rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs within cells, with unprecedented levels of sensitivity and specificity. These innovative techniques have proven invaluable for various clinical diagnostic procedures, whether it's quantifying chromosomes for prenatal diagnosis, identifying aneuploidy, detecting viral infections in their early stages, or uncovering rare messenger RNAs in cells. The book contains a total of xiii + 165 pages.

Seller Inventory # 15900

Bibliographic Details

• Title: PRINS and In Situ PCR Protocols (Methods in Molecular Biology: Volume 71)
• Publisher: Humana Press, Totowa, New Jersey
• Language: English
• ISBN 10: 0896033953
• ISBN 13: 9780896033955
• Binding: Softcover
• Edition: First Edition.
• Printing: First Printing
• Condition: Very Good
• Dust Jacket Condition: No Jacket
• Dimensions: 9W x 6.25H x 0.5D inches 9 inches width by 6.25 inches height by 0.5 inches depth

About this title

Synopsis

Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.

Review

...[a] splendid little book which covers the field quite well...Chapetr 16, which is a general review of in situ PCR written by Aidan Long and Paul Komminoth...is by far the best review of the approach...yet seen and should be mandatory reading for anyone intending to try in situ PCR. -FEBS Letters

"...useful to all those increasingly applying such techniques to in situ analysis...this book contains clear practical instructions that are easy to follow, and is well illustrated. It is essential for any laboratory contemplating exploiting this powerful technique."-SGM Quarterly