Plant Analysis : Comprehensive Methods And Protocols

Contents: 1. General Guidelines for Experimentation and Plant Analysis. 2. Digestion of Plant Samples for Nutrient Analysis. 3. Determination of Plant Nutrients. 4. Leaf Pigments and Chlorophyll Fluorescence. 5. Analysis of Organic Molecules in Plant Tissues.6. pH and Buffer Solutions. 7. Enzymes: Extraction and Assay. 8. Assay of Some Key Enzymes of Plants. 9. Photosynthetic Enzymes and Important Metabolites. 10. Enzymes of Infected Plants and Parasites. 11. Disease Resistance Related Estimations. 12. Growth Indices and Leaf Area. 13. Measurement of Photosynthesis, Respiration and Transpiration.14. Stress Indicators in Plants. 15. Determination of Plant Water Status. 16. Aerenchyma: Formation, Functions and Measurement.17. Electrophoresis. 18. Molecular Biology Techniques. 19. Determination of Polyamines and Phenolics by TLC and HPLC Techniques. 20. Histochemistry of Enzymes and Important Metabolites. 21. Methods of Root Studies. 22. Symbiotic Nitrogen Fixation Related Estimations in Plants. 23. Assay of Growth Hormones. Appendices. Subject Index. The book Plant Analysis Comprehensive Methods and Protocols is a complete laboratory manual for analytical methods and techniques in the field of Agriculture Plant Physiology Biochemistry and related Plant Sciences. Right from nutrient analysis in plants it covers estimations of macromolecules such as amino acids proteins nucleic acids and metabolites of fatty acid metabolism. Protocols for the assay of various enzymes of nitrogen metabolism ammonia assimilation, photosynthetic CO2-fixation, reactive oxygen species, carbohydrate phosphorus and energy metabolism have been elucidated in the book. Special emphasis has also been given to techniques on specific topics such as Electrophoresis Molecular Biology Histo enzymology Symbiotic Nitrogen Fixation and assay of plant growth hormones. Thus the present book is one stop solution for all important techniques and analytical methods for students and research workers engaged in plant sciences and agricultural research. (jacket).…