Polymerase Chain Reaction (PCR): Principle to Practices

Contents: 1. Introduction. 2. Primers. 3. Variants of PCR. 4. Applications of PCR. 5. Loop mediated isothermal amplification assay. 6. Suggested readings. This book deals with different advancement of Polymerase chain reaction (PCR) which was discovered by the eminent Scientist Kary Banks Mullis. The book starts with historical background and general information of PCR including primer designing methodologies. We also focused on various recent modified forms of PCR along with their principle to practices in molecular biology research. Further we also included a chapter on Loop mediated isothermal amplification, which is recently using to identify different pathogens. The book will be helpful for the scientists, academicians, as well as researchers to brush up their knowledge regarding the technique and to apply the knowledge gained through this book more vividly in the field of molecular biology. The book will also be helpful for the students to develop the concepts involved in different critical steps of this technology to design primers.…