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Bibliographic Details

Title: Protein Crystallography: A Concise Guide
Publisher: Johns Hopkins University Press
Publication Date: 2008
Language: English
ISBN 10: 0801888085
ISBN 13: 9780801888083
Binding: paperback
Edition: 1st Edition.
Condition: Good

About this title

Synopsis

The proteome remains a mysterious realm. Researchers have determined the structures of only a small fraction of the proteins encoded by the human genome. Crystallography continues to be the primary method used to determine the structures of the remaining unknown proteins. This imaging technique uses the diffraction of X-rays to determine a protein’s three-dimensional molecular structure.

Drawing on years of research and teaching experience, Eaton E. Lattman and Patrick J. Loll use clear examples and abundant illustrations to provide a concise and accessible primer on protein crystallography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals, phase determination (including MIR and MAD phasing and molecular replacement), the Patterson function, and refinement, Lattman and Loll provide a complete overview of this important technique, illuminated by physical insights.

The crisp writing style and simple illustrations will provide beginner crystallographers with a guide to the process of unraveling protein structure.

About the Authors

Eaton E. Lattman is chair of the Department of Biophysics at the Johns Hopkins University and editor-in-chief of the journal Proteins: Structure, Function, and Genetics.

Patrick J. Loll is the director of the Biochemistry Graduate Program at Drexel University College of Medicine.

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