Renal and Urinary Proteomics: Methods and Protocols

Visith Thongboonkerd

ISBN 10: 3527319743 / ISBN 13: 9783527319749

Published by Wiley-Blackwell, 2010

About this Item

Hardcover, xxxviii + 384 pages, NOT ex-library. Limited signs of gentle wear. A clean copy with unmarked text, free of inscriptions and stamps; firmly bound. Published without a dust jacket. -- Based on research and results from the international Human Kidney and Urine Proteome Project, this handbook and reference presents current methods and protocols for the analysis of human kidney and urine tract. The world's leading experts cover everything from basic techniques for routine analysis to advanced methods for difficult samples or analytes. Written with the practitioner in mind, all the methods and techniques are described with step-by-step protocols backed by valuable practical advice taken from real life experiments. -- Contents: Part One: Renal Proteomics: -- 1 Isolation and Enrichment of Glomeruli Using Sieving Techniques; 2 Isolation and Enrichment of Glomeruli Using Laser Microdissection and Magnetic Microbeads for Proteomic Analysis; 3 Isolation and Analysis of Thick Ascending Limb of Henle's Loop (TALH) Cells; 4 Proteomic Analysis of the Renal Inner Medulla and Collecting Ducts; 5 Proteomic Analysis of Mesangial Cells; 6 Proteomic Analysis of Podocytes; 7 Proteomics of Renal Peroxisomes; 8 Applications of Tissue Microarrays in Renal Physiology and Pathology; 9 Proteomic and Mass Spectrometric Analyses of Formalin-Fixed Paraffin-Embedded Tissue; 10 Selective Tissue Procurement for Renal Tumor Proteomics; 11 Proteomic Analysis of Primary and Established Cell Lines for the Investigation of Renal Cell Carcinoma; 12 PROTEOMEX Analysis of Renal Cell Carcinoma; 13 In Vivo Labeling of the Kidney by Means of CyDye DIGE Fluors Prior to Proteomic Analysis; Part Two: Urinary Proteomics: -- 14 Simple Methods for Sample Preparation in Gel-Based Urinary Proteomics; 15 Prefractionation of Urinary Proteins; 16 Gold Nanoparticle-Assisted Protein Enrichment for Urinary Proteomics; 17 Enrichment of Human Urinary Proteins Using Solid-Phase Extraction Column Chromatography; 18 Enrichment and Analysis of Concanavalin A-Captured Urinary Glycoproteins; 19 Isolation and Enrichment of Urinary Exosomes for Biomarker Discovery; 20 2-D Difference In-Gel Electrophoresis (2-D DIGE) and Urinary Proteomics; 21 Liquid Chromatography Coupled to Mass Spectrometry for Analysis of the Urinary Proteome; 22 Comparative Urine Analysis by Liquid Chromatography- Mass Spectrometry: Data Processing and Multivariate Statistics; 23 Surface-Enhanced Laser Desorption/Ionization for Urinary Proteome Analysis; 24 Capillary Electrophoresis Coupled to Mass Spectrometry for Urinary Proteome Analysis; 25 Mass Spectrometric Immunoassay in Urinary Proteomics; 26 Antibody Microarrays for Urinary Proteome Profiling; 27 Proteomic Analysis of Dialysate Fluid and Adsorbed Proteins on Dialysis Membranes During Renal Replacement Therapy; Index.

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Bibliographic Details

• Title: Renal and Urinary Proteomics: Methods and Protocols
• Publisher: Wiley-Blackwell
• Publication Date: 2010
• Language: English
• ISBN 10: 3527319743
• ISBN 13: 9783527319749
• Binding: Hardcover
• Edition: 1st Edition
• Condition: Good

About this title

Synopsis

Auf der Grundlage der Resultate, die das internationale "Human Kidney and Urine Proteome Project" vorlegte, fasst dieser Band moderne Methoden und Protokolle für die Analyse von Nierengewebe und Urin des Menschen zusammen. Schritt für Schritt, ergänzt durch praxistaugliche Tipps und Hinweise für die Laborarbeit, werden Routinemethoden ebenso ausführlich vorgestellt wie Spezialverfahren für kompliziertere Proben oder Analyte.

About the Author

Visith Thongboonkerd studied Medicine in Thailand and Japan, receiving an M. D. degree from Chiang Mai University (Thailand). He then moved to the University of Louisville (Kentucky, USA) as a research associate, becoming a member of the faculty in 2003. He is presently heading the Molecular Biology Research Unit at the Sirijay Hospital in Bangkok, Thailand. Dr. Thongboonkerd is a board member of the international "Human Kidney and Urine Proteome Project" (HKUPP) that is run under supervision of the Human Proteome Organization. He is also an editorial board member of several high impact journals in proteomics and nephrology.