This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward.

Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include:

Optical techniques, fluorescent probe design, and algorithm development
Recent highlight and breakthroughs in biology using SRM methods
The overall success of SRM in biological inventions
The future direction and scope of the field

This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.

About the Author

Dr. Junle Qu is a Professor in the College of Physics and Optoelectronic Engineering at Shenzhen University and the director of Center for Biomedical Optics and Photonics of Shenzhen University. His current research interests include biomedical optical imaging and imaging guided optical therapy. He has published more than 500 papers in peer reviewed journals.

Dr. Zhigang Yang is currently an associate Professor in the College of Physics and Optoelectronic Engineering, Shenzhen University and a member of Center for Biomedical Optics and Photonics of Shenzhen University. His research interests mainly focus on development of optical probes and biological imaging, including super-resolution fluorescent imaging, fluorescence lifetime imaging, and Visible-Near Infrared I/II imaging.

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