Editor(s): Lee, Kiho. Series: Methods in Molecular Biology. Num Pages: 288 pages, 10 black & white illustrations, 41 colour illustrations, biography. BIC Classification: MFN. Category: (P) Professional & Vocational. Dimension: 254 x 178. . . 2017. 1st ed. 2017. hardcover. . . . .

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Bibliographic Details

Title: Zygotic Genome Activation: Methods and Protocols: 1605 (Methods in Molecular Biology, 1605)
Publisher: Humana
Publication Date: 2017
Language: English
ISBN 10: 1493969862
ISBN 13: 9781493969869
Binding: Hardcover
Edition: 1st Edition
Condition: New

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Synopsis

This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.

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