Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell - Hardcover

Hardcover

ISBN 10: 038731234X ISBN 13: 9780387312347

Publisher: Springer, 2006

Synopsis

This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope.

The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.

About this title

From the Back Cover:

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.

This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author's field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.

About this edition

• Publisher: Springer
• Publication date: 2006
• ISBN 10: 038731234X
• ISBN 13: 9780387312347
• Binding: Hardcover
• Edition number: 2
• Number of pages: 470
• Editor: Frank Joachim