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Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology) - Softcover

 
9781071633755: Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology)

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9781071633724: Base Excision Repair Pathway: Methods and Protocols (Methods in Molecular Biology, 2701)

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ISBN 10: 1071633759 ISBN 13: 9781071633755
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Published by Springer US Aug 2024, 2024
ISBN 10: 1071633759 ISBN 13: 9781071633755
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 276 pp. Englisch. Seller Inventory # 9781071633755

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Published by Springer Verlag GmbH, 2024
ISBN 10: 1071633759 ISBN 13: 9781071633755
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Tapas K. Hazra
Published by Springer US, 2024
ISBN 10: 1071633759 ISBN 13: 9781071633755
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repairproteinsand copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway. Seller Inventory # 9781071633755

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