Ionic Channels in Cells and Model Systems - Softcover

Softcover

ISBN 10: 1468450786 ISBN 13: 9781468450781

Publisher: Springer, 2013

Synopsis

I. Methodologies.- 1 Kinetic Models and Channel Fluctuations.- 1. Introduction.- 2. Two-State Channel.- 3. Two Two-State Channels.- 4. Three-State Channel with Three Conductances.- 5. Three-State Channel with Only Two Conductances.- References.- 2 Single-Channel Currents and Postsynaptic Drug Actions.- 1. Introduction.- 2. Channel Gating as a Stochastic Process.- 3. Postsynaptic Channels in the Presence of Drugs.- 4. Reconstructing the Postsynaptic Current.- 5. Macroscopic and Molecular Consequences.- References.- 3 Voltage-Dependent Gating: Gating Current Measurement and Interpretation.- 1. Introduction.- 2. Voltage Gating.- 3. Gating Current Is a Capacitive Current.- 4. Measurement of Gating Currents.- 5. Gating of the Sodium Channel.- References.- 4 Characterizing the Electrical Behavior of an Open Channel via the Energy Profile for Ion Permeation: A Prototype Using a Fluctuating Barrier Model for the Acetylcholine Receptor Channel.- 1. Introduction.- 2. Theory.- 3. Confrontation with Experimental Data for the AChR Channel.- 4. Discussion.- References.- 5 The Use of Specific Ligands to Study Sodium Channels in Muscle.- 1. Introduction.- 2. Molecular Pharmacology of the Sodium Channel in Muscle.- 3. Sodium Channel in Cardiac Muscle: Are All Sodium Channels Alike?.- 4. Surface and Tubular Sodium Channels in Skeletal Muscle.- 5. Models for Sodium Channels in Muscle Membranes.- References.- 6 Isolation of Muscle Membranes Containing Functional Ionic Channels.- 1. Introduction.- 2. Excitation-Contraction Coupling.- 3. Ionic Channels and E-C Coupling.- 4. Isolation of Muscle Membranes.- 5. Concluding Remarks.- References.- 7 Methodologies to Study Channel-Mediated Ion Fluxes in Membrane Vesicles.- 1. Introduction.- 2. Channel-Mediated Tl+ Flux Measured by Fluorescence Quenching.- 3. Channel-Mediated Ion Fluxes Measured by Light Scattering.- References.- 8 Optical Studies on Ionic Channels in Intact Vertebrate Nerve Terminals.- 1. Introduction.- 2. Equivalence of Optical and Electrical Measurements of Membrane Potential.- 3. Optical Recording of Action Potentials from Nerve Terminals of the Frog Xenopus.- 4. Properties of the Action Potential in the Nerve Terminals.- 5. Ionic Basis of the Depolarizing Phase of the Action Potential.- 6. Concluding Remarks.- References.- 9 Optical Detection of ATP Release from Stimulated Endocrine Cells: A Universal Marker of Exocytotic Secretion of Hormones.- 1. Introduction.- 2. Methodological Considerations.- 3. Acetylcholine-Induced ATP Release from Chromaffin Cells: Calcium Dependence.- 4. Nicotinic Receptor Desensitization.- 5. Granular Nature of the Secreted ATP.- 6. ATP Release Evoked by Membrane Depolarization Is Mediated by Activation of Voltage-Gated Calcium Channels.- 7. ATP Release from Collagenase-Isolated Islets of Langerhans.- 8. Conclusion.- 9. Summary.- References.- II. Channels in Biological Membranes.- 10 Mechanotransducing Ion Channels.- 1. Introduction.- 2. Recording SA Channels.- 3. General Characteristics.- 4. Conductance Properties.- 5. Kinetic Properties.- 6. The Model.- 7. Comparing the Model to the Data.- 8. Future Prospects.- References.- 11 Ionic Channels in Plant Protoplasts.- 1. Introduction.- 2. Some Methodological Considerations.- 3. Voltage-Dependent Channels Opened by Hyperpolarization.- 4. Channels Affected by TEA.- 5. Conclusions.- References.- 12 Channels in Kidney Epithelial Cells.- 1. Introduction.- 2. Cell Culture.- 3. Patch-Clamp Methodology.- 4. Potassium Channel Characteristics.- 5. Channel Modulation.- 6. Conclusions.- References.- 13 Channels in Photoreceptors.- 1. Introduction.- 2. Vertebrate Photoreceptors.- 3. Invertebrate Photoreceptors.- References.- 14 Inactivation of Calcium Currents in Muscle Fibers from Balanus.- 1. Introduction.- 2. Methodological Considerations.- 3. Characteristics of Inward Currents.- 4. Mechanism of Inactivation.- References.- 15 Electrophysiological Studies in Endocrine Cells.- 1. Introduction.- 2. Whole-Cell Patch-Clamp M

Edition details

• Publisher: Springer
• Publication date: 2013
• Language: English
• ISBN 10: 1468450786
• ISBN 13: 9781468450781
• Binding: Paperback
• Number of pages: 464
• Editor: Latorre Ramón