Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art...
Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA...
From Reviews of the First Edition
"...continues the standard of excellent laboratory manuals that has been set by earlier volumes...Many of the protocols are significantly easier to follow than comparable protocols in other lab handbooks...provides one of the best collections of manuals for DNA sequencing applications..." -FEBS Letters
"...a valuable addition to the library of any laboratory that is actively engaged in DNA sequencing..." -Quarterly Review of Biology
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