Items related to A Laboratory Guide to Biotin-Labeling in Biomolecule...
Synopsis
ceptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .
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- PublisherBirkhäuser
- Publication date2012
- ISBN 10 3034873514
- ISBN 13 9783034873512
- BindingPaperback
- LanguageEnglish
- Number of pages254
- EditorMeier T., Fahrenholz F.
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Springer, Basel, Birkhäuser Basel, Birkhäuser Mrz 2012, 2012
ISBN 10: 3034873514
ISBN 13: 9783034873512
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 240 pp. Englisch. Seller Inventory # 9783034873512
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - 2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . Seller Inventory # 9783034873512
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
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