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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols - Hardcover
Synopsis
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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- PublisherSpringer
- Publication date2015
- ISBN 10 3319162586
- ISBN 13 9783319162584
- BindingHardcover
- LanguageEnglish
- Number of pages48
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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Seller: Lucky's Textbooks, Dallas, TX, U.S.A.
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Low-Cost Methods for Molecular Characterization of Mutant Plants (Hardcover)
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Springer International Publishing AG, Cham, 2015
ISBN 10: 3319162586
ISBN 13: 9783319162584
New
Hardcover
Seller: Grand Eagle Retail, Fairfield, OH, U.S.A.
Seller rating 5 out of 5 stars
Hardcover. Condition: new. Hardcover. This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Seller Inventory # 9783319162584
Quantity: 1 available
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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Seller: Revaluation Books, Exeter, United Kingdom
Seller rating 5 out of 5 stars
Hardcover. Condition: Brand New. 2015 edition. 35 pages. 9.40x6.20x0.40 inches. In Stock. Seller Inventory # x-3319162586
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Low-Cost Methods for Molecular Characterization of Mutant Plants
Published by
Springer International Publishing, 2015
ISBN 10: 3319162586
ISBN 13: 9783319162584
New
Hardcover
Seller: moluna, Greven, Germany
Seller rating 5 out of 5 stars
Gebunden. Condition: New. Open Access provided with a grant from the International Atomic Energy AgencyDescribes low-cost and easy-to-use assays for the molecular characterization of mutant plant materialThe experimental protocols do away with the need for. Seller Inventory # 31551099
Quantity: 3 available