Rapid Cycle Real-Time PCR: Methods and Applications (Springer Lab Manuals) - Softcover

Softcover

ISBN 10: 3540667369 ISBN 13: 9783540667360

Publisher: Springer, 2001

Synopsis

The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.

About this title

From the Back Cover

Rapid Cycle Real-Time PCR allows quantification and sequence analysis in less than 30 minutes. This powerful technique can be applied to any nucleic acid for rapid testing in microbiology, genetics, and oncology. Fluorescence analysis provides convenient determination of product of probe melting temperatures that allows product identification, even down to a single base.

Edition details

• Publisher: Springer
• Publication date: 2001
• Language: English
• ISBN 10: 3540667369
• ISBN 13: 9783540667360
• Binding: Paperback
• Edition number: 1
• Number of pages: 408
• Editor: Meuer S., Wittwer Carl, Nakagawara K.