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Prokaryotic Genomics (Methods and Tools in Biosciences and Medicine) - Hardcover

 
9783764365974: Prokaryotic Genomics (Methods and Tools in Biosciences and Medicine)

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This manual reflects practical approaches to handling bacteria in the labora- tory. It is designed to recall historical methods of bacterial genetics that have had recent developments and to present new techniques that allow full genome analysis. It has been written for microbiologists who need to group their protocols at the state of the art of a new millennium and also for scientists in other fields of life sciences who need to use bacteria for their research. Teachers, graduate students, and postdocs also will benefit from having these protocols to help them understand modern bacterial genetics. I learned so much from these contributions from my colleagues that I have no doubt about the daily usefulness of this book. April 2002 Michel Blot XII Abbreviations Acyl-HSL N-acyl homoserine lactone moi multiplicity of infection Amp or Ap ampicillin N amino C carboxy NMR nuclear magnetic resonance CIO-HSL N-decanoyl-L-homoserine lactone 3-0H-C14:1-HSL N-(3-hydroxy-7 -cis-tetra- C12-HSL N-dodecanoyl-L-homoserine lac- decanoyl)homo-serine lactone tone 3-0H-C4-HSL N-3-hydroxybutanoyl-L- C14-HSL N-tetradecanoyl-L-homoserine homoserine lactone lactone ONPG o-nitrophenyl -D-galactopyranoside C4-HSL N-butanoyl-L-homoserine lactone ORF open reading frame C6-HSL N-hexanoyl-L-homoserine lactone OTG I-S-octyl- -D-thioglucoside C8-HSL N-octanoyl-L-homoserine lactone 3-oxo-CIO-HSL N-3-oxodecanoyl-L-homo- Cam or Cm chloramphenicol serine lactone CBD chitin binding domain 3-oxo-C12-HSL N-3-oxododecanoyl-L- CHEF contour clamped homogenous electric homoserine lactone field 3-oxo-C14-HSL N-3-oxotetradecanoyl-L- CI consistency index homoserine lactone CRIM conditional-replication, integration, 3-oxo-C4-HSL N-3-oxobutanoyl-L-homoser- and modular ine lactone dCTP deoxycytidine triphosphate 3-oxo-C6-HSL N-3 -oxohexanoyl-L-homoser- deg.

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9783764365967: Prokaryotic Genomics

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ISBN 10:  376436596X ISBN 13:  9783764365967
Publisher: Birkhäuser, 2002
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Blot, Michel
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Hardcover. Condition: new. Hardcover. This text provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. The volume covers both revisited classical methods and comtemporary tools for global analysis such as genomics or proteomics. It is written for those in need of a bench manual to complete their experiments and for those wanting to understand the modern tools used in microbiology. It features: specific, step-by-step protocols for prokaryotic genomics and proteomics; genome mapping and DNA fingerprinting of bacteria; bacterial genetics, including methods to identify exchange and transfer genes; expression measures, genetic footprinting and microbial two-hybrid assay; protein purification with inteins; tips for data interpretation and useful websites; a troubleshooting guide; a guide to solutions; and a guide to protocols. Protocols are accompanied by notes and lab tricks and alternative methods are provided and discussed. The text is intended for: researchers working in microbiology, molecular and cell biology, biotechnology and biochemistry; biotechnology companies; and graduate and postgraduate students. Covering both revisited classical methods and contemporary tools for global analysis such as genomics or proteomics, this text provides molecular microbiologists in particular and researchers working with bacteria in general with the most important established recipes needed for their work. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Seller Inventory # 9783764365974

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Gebunden. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. THE BOOK DESCRIBES IMPORTANT TECHNIQUES FROM MODERN PROKARYOTIC GENETICS AND GENOMICS WITH EXTENSION TO METHODS FROM PROTEOMICS MIXTURE BETWEEN IMPORTANT ESTABLISHED AND TOPICAL METHODS NO RIVAL METHOLOGY BOOK KNOWN THAT COVERS PROKARYOTIC GENETICS, GENOMIC. Seller Inventory # 5279462

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