Systematic Analysis of Working Models to Detect Thioredoxin Activities: Detecting Thioredoxin Activity by Systemic Analytical Tools - Softcover

Verma, Neha; Abbot, Vikrant; Sharma, Mrinal

Softcover

ISBN 10: 6206844064 ISBN 13: 9786206844068

Publisher: LAP LAMBERT Academic Publishing, 2023

Synopsis

Thioredoxin (Trx) (12 kDa M.W.) is a ubiquitous antioxidant protein which controls the oxidative stress and redox signaling with thioredoxin reductase (TrxR) and NADPH. Trx acts as an electron donor for ribonucleotide reductase (RNR), methionine sulfoxide reductase (Msr), and Trx peroxidase (peroxiredoxin). Trx plays an important role in DNA synthesis, reduction of the disulfide bond, recycling of peroxiredoxin (Prx), and as an electron donor. Various in vivo and in vitro assays are used to check the activity of Trx. This work is focused on the study of in vivo and in vitro methods for Trx activity which have been used in the different model system. Different methods were studied to evaluate the activity of Trx in different samples. Insulin reduction assay is more commonly used in vitro method because insulin acts as effective substrate for activity of Trx. Fluorescence, DTNB (5, 5ʹ-dithiobis (2-nitrobenzoic acid)) reduction, spectrophotometric or micro-method insulin reduction, and disulfide cross-linking interaction in vitro assays while Luciferase, cloning, and wort in vivo assays are used for the activity of the Trx system.

Edition details

• Publisher: LAP LAMBERT Academic Publishing
• Publication date: 2023
• Language: English
• ISBN 10: 6206844064
• ISBN 13: 9786206844068
• Binding: Paperback
• Number of pages: 52