Application of Microarray technology: A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation.

Ray, Anamika

ISBN 10: 3838364066 / ISBN 13: 9783838364063

Published by LAP LAMBERT Academic Publishing, 2010

About this Item

LIKE NEW. SHIPS FROM MULTIPLE LOCATIONS.

Seller Inventory # ERICA80038383640666

Bibliographic Details

• Title: Application of Microarray technology: A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation.
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2010
• Language: English
• ISBN 10: 3838364066
• ISBN 13: 9783838364063
• Binding: paperback
• Condition: Like New
• Book Type: book

About this title

Synopsis

The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions.