Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus

Shrivastava, Neeraj, Sharma, Bhaskar, Shrivastav, Arpita

ISBN 10: 6200587884 / ISBN 13: 9786200587886

Published by LAP LAMBERT Academic Publishing, 2020

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Bibliographic Details

• Title: Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2020
• Language: English
• ISBN 10: 6200587884
• ISBN 13: 9786200587886
• Binding: paperback
• Condition: New
• Book Type: book

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Synopsis

The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF’ cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.