A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

From the Back Cover

This volume in the acclaimed seriesMethods in Cell Biology is designed for students and researchers in biology and biomedicine.A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca2+buffers appropriate to their research needs.
Highlights
* Describes techniques to
* Detect Ca2+with sensitive microelectrodes, fluorescent dyes, and luminescent proteins
* Perturb intracellular Ca2+
* Prepare Ca2+buffers with an easy-to-use computer program

* Contains color plates that illustrate confocal ratio-imaging and the use of aequorin
* Covers methods in detail and describes the problems and pitfalls of each technique

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