Strategies for Production of Single Chain Antibody Fragment in E. coli | Production of Single chain antibody fragment

Ganesh Patil

ISBN 10: 3659482897 / ISBN 13: 9783659482892

Published by LAP LAMBERT Academic Publishing, 2014

About this Item

Strategies for Production of Single Chain Antibody Fragment in E. coli | Production of Single chain antibody fragment | Ganesh Patil | Taschenbuch | 124 S. | Englisch | 2014 | LAP LAMBERT Academic Publishing | EAN 9783659482892 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.

Seller Inventory # 105456071

Bibliographic Details

• Title: Strategies for Production of Single Chain Antibody Fragment in E. coli | Production of Single chain antibody fragment
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2014
• Language: English
• ISBN 10: 3659482897
• ISBN 13: 9783659482892
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 9D millimeters 220 millimeters width by 150 millimeters height by 9 millimeters depth
• Weight: 203 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

Recombinant expression of antibody fragments in E. coli is often hampered by low expression, inefficient translocation and aggregation in the periplasm. Aggregation problem during in vitro-renaturation is also a limiting factor, when antibody fragments expressed in bacterial cytosol as inclusion bodies. The objective of this work was to achieve and optimise periplasmic production, and to explore novel strategies for in vitro renaturation from cytoplasmic IB’s. For the study, the single chain antibody fragment against the hapten oxazolone was chosen as the model system. In this book, results on periplasmic expression and expression as inclusion bodies and refolding thereof are presented. In expression in periplasmic effect of promoter strength and growth cultivation are presented. In vitro renaturation of IB's lead to establishment refolding protocols comprising of Ionic Liquids, which are emerging as green alternatives. Besides, we demonstrated usage of aromatic thiols as redox buffers components for protein refolding. Findings presented herein might be helpful for the of the periplasmic production of antibody fragments as as well as for establish protocols at physiological pH.

About the Author

Ganesh Patil has completed his Ph.D from Martin Luther University, Halle, Germany. His expertise lies in the area of recombinant protein cloning, expression, purification, characterization. He is specialized in the area of process development on production of recombinant proteins, bacterial and viral antigens and monoclonal antibodies.