PCR 3: PCR In Situ Hybridization: A Practical Approach (Practical Approach Series, 186)

ISBN 10: 019963632X / ISBN 13: 9780199636327

Published by Oxford University Press, 1998

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Bibliographic Details

• Title: PCR 3: PCR In Situ Hybridization: A Practical Approach (Practical Approach Series, 186)
• Publisher: Oxford University Press
• Publication Date: 1998
• Language: English
• ISBN 10: 019963632X
• ISBN 13: 9780199636327
• Binding: Soft cover
• Edition: 1st Edition
• Condition: New

About this title

Synopsis

PCR 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

About the Author

C. S. Herrington is at University of Liverpool. J. J. O'Leary is at John Radcliffe Hospital, Oxford.