On the Removal of Diffusible Substances from the Circulating Blood of Living Animals by Dialysis: II. Some Constituents of the Blood (Classic Reprint) - Softcover
Synopsis
Explore early blood chemistry and the quest to map its dissolved substances.
This edition presents a detailed look at how researchers studied the constituents that travel through the blood and diffusion systems. It covers methods for separating and identifying nitrogenous compounds, including amino acids, urea, and related substances, through a sequence of careful experiments and analyses.
- Learn how the scientists collected and purified dialysates, drew conclusions from diffusion rates, and used precipitation with phosphotungstic acid to isolate nitrogenous substances.
- See which amino acids emerged as major components, notably alanine, and note the absence of glycine in the samples described.
- Discover the handling of non-amino acids like lactic and oxybutyric acids, and the discussion around creatinine and related substances in the context of blood chemistry.
- Understand some of the experimental challenges and refinements, such as issues with ester distillation, sensitivity to reagent strength, and steps to minimize contamination.
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