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Methods for Transfecting Cells with Nucleic Acids of Animal Virusses: a Review.
Published by Birkhäuser Verlag, Basel, Stuttgart, 1971
Seller: Buchversand Joachim Neumann, Neubrandenburg, MV, Germany
Seller rating 5 out of 5 stars
Experientia, Supplementum 16. pp 82.
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Methods for Transfecting Cells with Nucleic Acids of Animal Viruses: a Review (Experientia Supplementum, 16) (German Edition)
Seller: Ria Christie Collections, Uxbridge, United Kingdom
Seller rating 5 out of 5 stars
US$ 60.27
US$ 15.85 shipping
Ships from United Kingdom to U.S.A.Quantity: Over 20 available
Add to basketCondition: New. In German.
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Condition: New. pp. 84.
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US$ 79.76
US$ 13.23 shipping
Ships from United Kingdom to U.S.A.Quantity: 2 available
Add to basketPaperback. Condition: Brand New. 82 pages. German language. 9.70x6.70x0.24 inches. In Stock.
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US$ 109.03
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Add to basketPaperback. Condition: Like New. Like New. book.
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Condition: Sehr gut. Zustand: Sehr gut | Sprache: Deutsch | Produktart: Bücher | Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from the infected tissue. To this end, procedures are designed to minimize the possibili ties of degradation of the viral nucleic acid by nucleases present in the biolo gical source. The most common method for preparing viral nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method shows astonishing 'cell-specificity'; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds.
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Condition: New. Print on Demand pp. 84 67:B&W 6.69 x 9.61 in or 244 x 170 mm (Pinched Crown) Perfect Bound on White w/Gloss Lam.
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Condition: New. PRINT ON DEMAND pp. 84.
